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Revision as of 09:21, 7 September 2010

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Bioinformatics for molecular biology - Fall 2010

Description

The aim of the course is to introduce students to bioinformatics resources and tools for molecular biology research by having some of the best researchers in Norway to talk about their field in general and then present their own work. Students are encouraged to bring a lap-top; we will be set up for in-course demonstrations as well as practical lab exercises. The course is intended for biology students or computer science/math students. No prior background in bioinformatics or computer science is required.

The course is jointly delivered by the Biotechnology Centre of Oslo, the Department of Molecular Biosciences (IMBV), the Department of Informatics (IFI) and the Norwegian University of Life Sciences. This course is one of the Ph.D. School courses offered by the Biotechnology Centre of Oslo (http://www.biotek.uio.no/ny-web/events/). The UiO page for this course is http://www.uio.no/studier/emner/matnat/molbio/MBV-INF4410/.

Registration will be open in May of 2010. Check back here for details

MBV-INF 4410 (M.Sc. level course code)10.0 study points

MBV-INF 9410 (Ph.D. level course code) 10.0 study points

MBV-INF 9410A (Ph.D. level course code) 8.0 study points

The course consists of two weeks of lectures, a final take-home exam (one week) and an essay (M.Sc. level 10 pages and Ph.D. level 20 pages to be completed by end of October).

Ph.D. level students may opt to take the course without the essay for only 8 study points.

Please bookmark this page. All future changes or announcements for the 2010 course will be posted to this page.

            Information: 
                 ragni.indahl@biotek.uio.no (about course administration) 
                 ian.donaldson@biotek.uio.no (about course content)
            Registration: 
                 torill.rortveit@imbv.uio.no

Dates and times

The course will occur September 6th to 17th.

Each day will consist of three time slots for lectures and/or practical labs between 9 AM and 4 PM.

Place

Forskningsparken

Directions to Forskningsparken can be found at http://www.biotek.uio.no/map.html

Mondays to Friday morning: Room 10 (one level down from the main entrance) The room is booked each day from 09:00 to 16:00 EXCEPT Fridays when the room must be vacated from 12 to 13:30.

Exceptions: none so far

Lunch is on your own between 1:00 PM and 2:15 PM. Please completely vacate the room during these lunch times.

Programme

Please note:
The schedule displayed below is based on last years course 
(see http://donaldson.uio.no/wiki/Bioinformatics_for_molecular_biology_2009).
Ongoing changes will be made to this page as we organize speakers over the next few months.  
Requests and suggestions are welcome.
Bioinformatics 2010
Time Speaker Contact Details and suggested readings
Day 1 - Monday September 6
9.00-9.15 Ian Donaldson http://donaldson.uio.no Introduction
9.15-11.00 Ian Donaldson http://donaldson.uio.no

Exploratory data analysis Extra material

11:15-13.00 Bjørn-Helge Mevik http://www.usit.uio.no/suf/vd/

Introduction to R - Presentation

14.15-16.00

Bjørn-Helge Mevik

Katerina Michalickova

http://www.usit.uio.no/suf/vd/

Introduction to R - Lab

Day 2 - Tuesday September 7
9.15-11.00 Clara-Cecilie Günther http://www.nr.no/

An introduction to statistical inference

11:15-13.00 Einar A. Rødland http://folk.uio.no/einarro/

Multiple hypothesis testing

14.15-16.00 Ian Donaldson http://donaldson.uio.no Installation and tutorial for MySQL
Day 3 -Wednesday September 8
9.15-11.00 Leonardo Meza-Zepeda Dept. of Tumor Biology, Rikshospitalet-Radiumhospitalet Medical Center

Microarray technology 1

Microarray technology 2

11:15-13.00 Ståle Nygård http://core.rr-research.no/index.php?section=22

Microarray data analysis

14.15-16.00 Ståle Nygård R Microarray data analysis lab
Day 4 - Thursday September 9
9.15-11.00 Torbjørn Rognes http://www.rr-research.no/rognes/

Searching sequence databases and multiple sequence alighments.

11:15-13.00 Geir K. Sandve http://www.uio.no/sok?la=en&person=geirksa Motif scanning and discovery in DNA
14.15-16.00

Geir K. Sandve

Halfden

Vegard Nygaard

Sequence lab

RSAT

ConTra

Day 5 - Friday September 10
9.15-11.00

Sveinung Gundersen

Geir K. Sandve

Hyperbrowser tutorial

http://hyperbrowser.uio.no/

11.15-12:00 Dave W. Ussery http://www.cbs.dtu.dk/staff/dave/ Comparative genomics

Methods to compare genomes

12.00-13:30 Lunch -vacate room
13:30-14:15 Dave W. Ussery http://www.cbs.dtu.dk/staff/dave/ Comparative genomics

Pan-genomics and metagenomics

14.15-16.00 Sequence lab
Day 6 - Monday September 13
9:00-11:00 Robert Lyle

High throughput sequencing

11:15-13.00 George Magklaras (TBC) http://www.no.embnet.org/

EMBNet

13:00-14:00 Lunch
14.15-16.00 Kamran Shalchian-Tabrizi (TBC) http://www.bioportal.uio.no

Bioportal_and_Eukarytic_megaevolution.pdf

Day 7 - Tuesday September 14
9.15-11.00 Jon K. Laerdahl

http://folk.uio.no/jonkl/

http://www.rr-research.no/rognes/

http://www.bioinformatikk.no/

Structural biology review

PyMOL

Installing PyMol

11:15-13.00 Jon K. Laerdahl

Structural bioinformatics tools, predictors, and 3D modeling

14.15-16.00 Jon K. Laerdahl Structural tutorials and exercises

PyMol demonstration

PyMol excercise

Day 8 - Wednesday September 15
9.15-11.00 Jon K. Laerdahl Structural modeling tutorial
11:15-13.00 Jon K. Laerdahl Structural modeling tutorial
14.15-16.00 Jon K. Laerdahl Structural modeling tutorial
Day 9 - Thursday September 16
9.15-11.00 Ian Donaldson http://donaldson.uio.no

Working with gene lists and over-representation analysis

11:15-13.00 Ian Donaldson http://donaldson.uio.no

Interaction data resources

iRefIndex talk at BioPathways talk

14.15-16.00 Sabry Razick, Paul Boddie http://donaldson.uio.no Interaction lab

Install CytoScape and the iRefScape plugin. See http://cytoscape.org and http://irefindex.uio.no/wiki/README_Cytoscape_plugin_0.9x

Day 10 - Friday September 17
9.15-10.00
10.15-11.00 Arne Gjuvsland http://www.cigene.no/people.html

Causally cohesive genotype-phenotype modelling - systems biology meets genetics

11.15-12.00 Jon Olav Vik http://www.cigene.no/people.html

cGP map of the cardiomyocyte

12.00-13:30 Lunch -vacate room
13:30-14:15 Katerina Michalickova

http://hpc.uio.no/index.php/Main_Page

http://www.usit.uio.no/suf/vd/

TITAN cluster for beginners
14.30-15.15
15.15-16.00

Written assignment

Students enrolled in MBV-INF 4410 must complete a written assignment as part of the course requirements.

The assignment is due by Friday, October 29th. It should be emailed to ian.donaldson at biotek.uio.no preferably as a PDF document (Microsoft Word or OpenOffice is also acceptable). The assignment is to be between 1000 to 2000 words and no more than 10 pages. Topics include:


1) write an explanation of three or more methods that were covered in the course. These should be simple explanations aimed at someone approaching the topic for the first time. Your explanation may include derivations of equations (if they are clearly explained), figures or tables. Use examples. Describe how the concept can be applied to a problem in biological research and what limitations the method has. List any resources you use as well as references to additional material that a student might use if they want to follow up on the topic further. Please indicate whether your material may be used on the course's wiki page.


2) describe how you would use two or more of the methods covered in the course in your own research. Your proposal may include figures or tables. Give a short introduction to your problem area, clearly state your hypothesis and how you think it might be addressed by each of the methods. Provide justifications for your proposal as well as expected outcome. Describe potential risks (say, the method provides no meaningful results) and what you would do to mitigate this risk. List any resources you use.


3) you may define your own alternative topic. Please send an email to ian.donaldson at biotek.uio.no to have your topic approved first.

Exam

Please note:

The exam for this course will be a one week take home exam.  

That will begin directly after the lectures end.  

The exam must be handed in Monday, September 27th by 5 PM.

Bioinformatics links relevant to the course

Bioinformatics Links 2010
Name URL Description
Day 1-2: Statistics
StatSoft textbook http://statsoft.com/textbook/stathome.html Good overview of methods and concepts
SAS manuals http://support.sas.com/onlinedoc/913/ Thorough overview of analysis procedures found in SAS
GraphPad http://graphpad.com/help/prism5/prism5help.html?usingstatistical_analyses_step_by_s.htm See the GraphPad statistical guide for easy introductions to many concepts in statistics
R http://cran.r-project.org/ The Comprehensive R Archive Network

Introduction to R

Exploratory data analysis

Hypothesis testing

http://bioinformatics.ca/workshops/2009/course-content See the CBW course on "Exploratory Data Analysis Essential Statistics using R" at the bottom of this page. Slides and lecture recordings from Modules 1-3 cover much of the same material covered in the first two days of this course.
Learning R http://www.ploscompbiol.org/article/info:doi%2F10.1371%2Fjournal.pcbi.1000482 A Quick Guide to Teaching R Programming to Computational Biology Students
R reference card http://cran.r-project.org/doc/contrib/Short-refcard.pdf A reference card for R syntax
Which test do I use http://www.practicalstats.com/which/index.html An interactive guide to choosing which statistical test to use.
EMBNet Microarray course http://vit-embnet.unil.ch/CoursEMBnet/Arrays06/Material.html An online course with R/Bioconductor example tutorials
EMBNet tutorials http://www.ch.embnet.org/pages/courses2.html Other helpful tutorials with R examples related to biostatistics
Day 3 - Microarrays
NMC http://www.mikromatrise.no/ Norwegian Microarray Consrtium
MACF http://core.rr-research.no/ UiO MicroArray Facility
Bioinformatics core facility http://core.rr-research.no/index.php?section=3 The Bioinformatics Core Facility established at Rikshospitalet-Radiumhospitalet (RR) will provide its users at RR and the University of Oslo with a range of services within bioinformatics, including analysis of DNA and protein sequences, analysis of microarray data, protein structure analysis and access to useful databases and web services
Day 4 - 5 Sequence
RSAT RSAT A collection of several motif-related tools
ConTra ConTra A more user-friendly tool for matching a collection of Position Weighted Matrices against promoter sequences across species
Day 6 -
Day 7 -
Required files [Sample network[1]] [Node atributes[2]] Please download these and uncompress before using
Cytoscape http://www.cytoscape.org/ Cytoscape home page
Cytoscape http://cytoscape.org/cgi-bin/moin.cgi/Presentations Tutorial on Cytoscape
iRefIndex Cytoscape plugin http://irefindex.uio.no/wiki/README_Cytoscape_plugin_0.5x iRefIndex installation
iRefIndex http://irefindex.uio.no iRefIndex wiki
iRefIndex publication http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=18823568 Full details of iRefIndex
DAVID http://www.nature.com/nprot/journal/v4/n1/pdf/nprot.2008.211.pdf Nature Protocols paper
GSEA http://www.broadinstitute.org/gsea/doc/subramanian_tamayo_gsea_pnas.pdf PNAS paper
GSEA http://www.broadinstitute.org/gsea Gene Set Enrichment Analysis application

Bioinformatics Mailing Lists

If you are interested in being informed of future courses, talks and new related to bioinformatics in the Oslo region, then consider signing up for the cbo mailing list.

https://sympa.uio.no/usit.uio.no/info/cbo-all

You might also consider the Norwegian-wide bioinformatics email list. You can sign up at

http://mailman.uib.no/listinfo/bioinfo.users.

Both lists are run by members of the Norwegian Bioinformatics Platform.