iRefScape plugin menu
- 1 Search tools
- 2 Loading user variables (this section is being edited)
- 3 iRefScape search with batch file
- 4 Only variables
- 5 View tools
- 6 Hide/Un-hide
- 7 Safe mode
- 8 All iRefIndex Pages
Retrieve interactions for the selected nodes
To load all protein-protein interactions and complexes involving one or more selected nodes. This action is the same as performing a iteration=1 search using iRefScape for the selected nodes.
Expand one level
To load all protein-protein interactions and complexes involving at least one of the nodes in the current network. The behaviour is same as performing iteration=1 search using iRefScape for all the nodes.
Load interactions between neighbours
To complete the interactions between the currently loaded nodes. When a search & load operation is performed in iRefScape, the network returned contains all the proteins interacting with the query and the corresponding interactions. This does not return interactions between the neighbours of the query. This operation is the same as performing an iteration=0 search using iRefScape for all the nodes. This action has no effect for networks for which this operation has already been performed using the option presented at the end of the search.
Clear load history (Reload everything next time)
This will clear the iRefScape cache memory containing currently loaded nodes and edges. IRefScape keeps track of what is loaded to avoid reloading and thus increase efficiency. Therefore, if a search is performed twice,the network will not be re-loaded unless the user has requested that results be shown in a new view or they have selected this menu option. If the user wishes to reload the network then this option has to be used. When using other methods of expanding the network like “Expand one level” iRefScape handles the cache clearing automatically.
Reset node degree
This option resets the i.alive_degree of nodes. i.alive_degree provides how many nodes with a certain attribute type is connected to each node (set using filters).
Loading user variables (this section is being edited)
User variables can be attached to nodes and be browsed using the attribute browser. This file can not be used as a searchable index (can not be used as a search type). please see section XXX for details on searchable indices.
There are three ways to load own variables for the user.
- Use the Cytoscape attribute loading feature (Cytoscape manual)
- Use a use attribute file with iRefScape (described in this section)
- Include the attribute with the batch search file.(described in XXX).
The iRefScape user attribute loader is provided as a complementing feature to the Cytoscape node attribute loading (Cytoscape manual). For most of the attribute loading operations the Cytoscape node attribute loader is sufficient and easier to use. However, when the iRefScape node attribute file is used, this can be used by just using the menu entry XXX. In addition the file will be available for subsequent operations.
iRefScape user variable file format
The first line of the file provides instructions and this line is compulsory.
Example. #i.rog New_attribute
The first line always starts with a hash ("#") and immediately followed by the iReFscape variable name. This iRefScape variable name is used to locate the nodes via the new attribute will be attached. Then a tab is used as a column separator, followed by the name of the user attribute name.This user attribute name should be unique( not already used). In the above example the user attribute to use is the i.rog and the user attribute name is "New_attribute".
From the second line onwards, the attributes with the mapping attribute are provided.
Example 4399398 TENC1|UniProtKB:Q63HR2|
The first column provides the iRefScape attribute to use and the second column provides the user attribute. Multiple attributes should be separated with a pipe ("|"). The example line shown would locate the node with i.rog=4399398 and attach the "TENC1|UniProtKB:Q63HR2|" value to it with the attribute name "New_attribute"
- Each column is separated by a TAB.
- Each line ends with a new line character ("\n")
- The file should be constructed using a plain text editor (e.g. Kate, notepad) and word processing software like Microsoft word should not be used to construct this file.
- The file extension is ".txt"
- Sample file "ONE__EXT__ROG_#Name_5_.iruv" can be found in the iRefScape installation directory (iRefIndex directory)
Sample file #i.rog New_name 4399398 TENC1|UniProtKB:Q63HR2| 452957 GFI1B|UniProtKB:Q5VTD9| 561552 TENC1|UniProtKB:Q63HR2-4| 534065 GFI1B_MOUSE|UniProtKB:O70237|
iRefScape search with batch file
When there are many search queries, it is convenient to construct a batch file and load this to iRefScape. There are 3 type of batch files;
- Simple batch file (recommended for users just wanting to search for a list of things)
- Attribute batch file (Attach additional attributes)
- User index batch file (please refer XXX)
Simple batch file
Example file content: #geneID:3702 814707 814714 814714 817659 818662
- The first line starts with a hash ("#") and this line is compulsory.
- Immediately after the hash (no space in between) the iRefScape attribute name should be provided. Please see below for accepted list of identifier with the corresponding label.
|Label to use in the batch file||Description||'||'||'|
|rog||iROGID, the integer reference redundant object group identifier(\'\'\'details of mapper files to locate the iRGOID can be found here)\'\'\'|
|geneID||NCBI Gene ID, this is always an integer|
|geneSymbol||NCBI Gene symbol|
|src_intxn_id||Interaction identifiers used by source databases|
|omim||OMIM identifier (\'\'\'OMIM home page\'\'\')|
|digid||diseases group identifier (\'\'\'DiG home page\'\'\')|
Users can load there own variables for proteins which are mapped to a ROGID. The file should follow the format given below;
- Should be a plain text file with exactly two columns and each line ending with a new line character (pressing enter).
- First column should contain the ROGID
- Second column should contain the user attribute/variable.
- The file name should have the format _rog_USER_VARIABLE_NAME.iruv (The text in bold is supplied by the user. e.g., _rog_myAttribute.iruv ).
e.g #rog USER_VARIABLE j4H4IZH/ecTvEDjCel+eLTCmFyE9606 1 j4H4IZH/ecTvEDjCel+eLTCmFyE9606 1 q6/pRFyay7gQ6bb2rzvKYgfkeaE9606 2 8sODZSppCsuEgRmhSHp3UjwOf2M9606 3 obze7v7i2yTIIcJjd7laIriNFg89606 4 Ty0OLkmhoUPjUuLQQv5ykN057oM9606 5 4bhW4sEt1YhYwHnHCyDNV3vvThs9606 6 Al8M+JdB4H+QnwITh8KcicdI2x49606 6 rkL/SGL1Enj6KoFkPbbcoJ5vKJE9606 6
Toggle selected multi-edges
iRefScape creates multiple edges between nodes to represent multiple evidences. If an interaction between two proteins has evidence from both MINT and IntAct, there will be 2 edges; one for MINT and one for IntAct. Although this provides more information for the user, sometime the user may prefer to collapse these edges to a single edge to facilitate viewing. When this action is performed on a network, all but a randomly selected edge between each node is hidden. The edges which are hidden has the i.flag=1 and the one remaining has i.flag=0. Therefore, while this process is random by default, the user can control which edges to keep and which to hide by editing the i.flag edge attribute before using the toggle multi-edge option.
Zoom to selected
This action brings the selected node(s) to the centre of the screen and makes the nodes as large as possible given that all selected nodes are visible. This action can also be performed manually using Cytoscape's zoom tool.
Select last iRefScape selection
This action shows the last selection performed by an iRefScape operation. This features does not record user selections or selections made by using Cytoscape tools (e.g filters).
Select nodes with different taxid than query node
Some interactions may involve proteins from multiple species. An interaction may truly occur between proteins from two species (e.g. an interaction between a Human protein and a virus protein). In other cases, this may be an artefact of how data was entered by the source database.
Select between nodes
This option highlights (selects) nodes interacting with at least two nodes that have been pre-selected by the user. The node attribute i.alive_degree will be recalculated to reflect the degree of each node with respect to this pre-selection.
Show spoke-represented complexes
Some complexes are represented as a set of binary interactions by some sources. This action attempts to highlight such complexes as a single unit by attaching them all to a single pseudonode.
Hide selected nodes
This action hides the selected nodes. This operation is performed in a independent thread and more efficient than hiding using Cytoscape's 'hide node' utility for large networks.
Hide nodes (except pseudonodes)
This action hides all select nodes which represent proteins. This will not hide pseudonodes representing a complex, a collapsed area or a collapsed hub.
Hide nodes not selected
Hides all nodes which are not currently selected
Hide nodes not selected (except pseudonodes)
Hides all nodes which are not currently selected and that represent proteins. This will not hide pseudonodes representing a complex, a collapsed area or a collapsed hub.
Un-hides all nodes which are hidden by the user.
This option is only available when using JAVA 1.5 and will not appear for Java 1.6 or newer. Selecting this will un-dock the iRefScape window and handles errors and exceptions thrown due to bugs in JAVA 1.5. When this mode is activated not all graphic features will be available.
All iRefIndex Pages
Follow this link for a listing of all iRefIndex related pages (archived and current).